Bacteriofago t4 pdf
T4 bacteriophage (phage)-infected cells show a marked increase in latent-period length, called lysis inhibition, upon adsorption of additional T4 phages (secondary adsorption). Lysis inhibition is a complex phenotype requiring the activity of at In addition, this method can be applied to a wide variety of pathogens including bacteria, phage, and human viruses. Using the bead transfer method, we demonstrate that an engineered micropattern limits transfer of Staphylococcus aureus by 97.8% and T4 bacteriophage by 93.0% on silicone surfaces. Allen EF, Albrecht I, Drake JW. Properties of bacteriophage T4 mutants defective in DNA polymerase. Genetics. 1970 Jun; 65 (2):187–200. [PMC free article] Barry J, Alberts B. In vitro complementation as an assay for new proteins required for bacteriophage T4 DNA replication: purification of the complex specified by T4 genes 44 and 62. Download PDF Download Full PDF Package. This paper. A short summary of this paper. 37 Full PDFs related to this paper. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Download. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. K P T4, aphage-coded thioredoxin, T4thioredoxin, is produced. In the phage-infected cells T4 thioredoxin brings about an electron flowfromNADPHvia thebacterial thioredoxin re-ductasetoaphage-specific ribonucleotide reductase(2). Apartfromthethioredoxin systemthereexists anotherre-cently discovered hydrogendonorsystem for ribonucleotide Montag, D.; Schwarz, H.; Henning, U., 1989: A component of the side tail fiber of Escherichia coli bacteriophage lambda can functionally replace the receptor-recognizing part of a long tail fiber protein of the unrelated bacteriophage T4
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure. Escherichia virus T4 is a species of bacteriophages that infect Escherichia coli bacteria. It is a double-stranded DNA virus in the subfamily Tevenvirinae from the family Myoviridae.T4 is capable of undergoing only a lytic lifecycle and not the lysogenic lifecycle.The species was formerly named T-even bacteriophage, a name which also encompasses, among other strains (or isolates ... infection. T4 tail is surrounded by a contractile sheath, which contracts during infection of the bacterium. At the end of the tail, phages like T4 have a base plate and one or more tail fibers attached to it. The base plate and tail fibers are involved in the binding of the phage to … Escherichia coli or Pseudomonas aeruginosa 48 h biofilms were challenged with phage T4 or PB-1, respectively, in combination with tobramycin. At 6 h and 24 h post challenge, total cells, tobramycin resistant cells, and phage resistant cells were determined. Key words: bacteriophage T4, fibritin, folding, foldon, superhelical protein, coiled coil Fibritin of bacteriophage T4 is a fibrous superhelical protein which consists of three identical polypeptide chains forming a parallel, segmented, α-helical struc-ture; super helical (coiled-coil) parts are separated by extended loops .
Bacteriophage T4 Escherichia coliis one of the pri-mary tools of molecular biology; it is widely used for studying the mechanisms controlling assembly and mor-phogenesis of complex biological structures [1, 2]. The principles involved in T4 assembly can be encountered in a broader range of cell biology processes including The Sequence of steps in bacteriophage T4 assembly has been elucidated by using a combination of genetic, biochemical, and ultrastructural techniques. The phage head, tail, and tail fibers are assembled via independent pathways, and then are joined to form the complete virus. Current knowledge of these three patways is reviewed briefly. Two general insights emerging from phage assembly studies ... Summary We have developed a system of transposon mutagenesis for bacteriophage T4. The transposon is a plasmid derivative of Tn5 which contains the essential T4 gene 24, permitting a direct selection for transposition events into a gene 24‐deteted phage. The transposition occurred at a frequency of only 10‐7 per progeny phage, even though a dam‐ host was used to increase … Previous genetic and biochemical analyses have established that the bacteriophage T4-encoded Gp31 is a cochaperonin that interacts with Escherichia coli ’s GroEL to ensure the timely and accurate folding of Gp23, the bacteriophage-encoded major capsid protein. The heptameric Gp31 cochaperonin, like the E. coli GroES cochaperonin, interacts with GroEL primarily through its unstructured mobile ... The aim of this study was to develop a minimal medium for the cultivation of Escherichia coli B, which could be especially suitable for the industrial propagation of bacteriophage T4. The new defined, minimal SM-1 culture medium, contains free amino acids as the only nitrogen source and enables the bacteria generation time to be prolonged and satisfactory phage titers to be achieved. T4 bacteriophage is a double-stranded DNA virus that consists of a hemi-icosahedral head, a cylindrical tail, and short and long tail fibers, which together form mature virus particles (11). Consisting of 40 structural proteins and containing 274 open reading frames, T4 bacteriophage has a diverse range of hosts, including E. coli K-12 (11 T4 r mutants are known as 'rapid lysis' mutants, creating larger virus plaques than non-r virus strains. T4rII mutants of T4r viruses are capable of using E. coli B as a host, but E. coli K12(l) is not a permissive host. When same dilutions are plated on E. coli B as on E. coli K12(l), very few, if any, plaques will be seen on E. coli K12(l), while many plaques will be observed on E. coli B. T4 phage was chosen for this experiment because the development of T4 phage in E. coli is well documented and T4 phage is commonly used in the study of bacteriophage infections of E. coli. E. coli B23, T4 bacteriophage, and wild-type Bacillus subtilis WB476 were provided from the MICB 421 culture collection of the Department of Microbiology and
Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases S Dong, HD Shew, LP Tredway, J Lu, E Sivamani, ES Miller, R Qu Transgenic research 17 (1), 47-57 , 2008 Kondabagil K. Bacteriophage mediated biocontrol in wastewater management. In Industrial and Environmental Microbiology, Studium Press, Chapter 30: 473-495, 2014 . Abdulrahman Al-Zahrani*, Kiran Kondabagil*, Song Gao, Noreen Kelly, Manjira Ghosh-Kumar, and Venigalla B. Rao1 The Small Terminase, gp16, of Bacteriophage T4 is a Global Regulator of ...
The Mre11-Rad50 complex (MR) from bacteriophage T4 (gp46/47) is involved in the processing of DNA double-strand breaks. Here, we describe the activities of the T4 MR complex and its modulation by proteins involved in homologous recombination. T4 Mre11 is a Rad50- and Mn2+-dependent dsDNA exonuclease and ssDNA endonuclease. ATP hydrolysis is required for the removal of multiple … Bacteriophage T4 and its host bacterium, Escherichia coli, can be considered among the earliest model organisms – biological systems that attract large numbers of investigators who, because of technical or conceptual advantages of the system, use the system to investigate processes and mechanisms of general significance in biology.Beginning in the mid‐twentieth century, studies focused on ... METHYL METHANESULFONATE MUTAGENESIS IN BACTERIOPHAGE T4 John W. Drake 1 1 Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709 MMS induces diverse rII mutations from a wild-type background in bacteriophage T4. About 56% are base pair substitutions, about 30% are frameshift mutations, and the remainder is a miscellaneous … Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0. entitled THE ECOLOGY OF BACTERIOPHAGE T4 -----and recommend that it be accepted as fulfilling the dissertation requirement for the Degree of Doctor of Philosophy f( ~ r/r12. Date 4/6/90 _ 1/r;,/y[) Date 4/6/96 Richard Michod Y/b)1J Date 4/6/90 Date Final approval and acceptance of this dissertation is contingent upon the ... US20100285482A1 US12/786,053 US78605310A US2010285482A1 US 20100285482 A1 US20100285482 A1 US 20100285482A1 US 78605310 A US78605310 A US 78605310A US 2010285482 A1 US2010285482 A1 US 2010285482A1 Authority US United States Prior art keywords polymerase method nucleic acid bacteriophage t4 recombination Prior art date 2001-04-20 Legal status (The legal …  Vinson C. G. & Petre A. W. Mosaic Disease of Tobacco. Chicago Journals [Internet]. 1929 [cited February 1929]; 87(1): [14-38]. Available from http://www.jstor.org ... Studies in the 1960s implied that bacteriophage T4 tightly couples DNA replication to genetic recombination. This contradicted the prevailing wisdom of the time, which staunchly supported recombination as a simple cut-and-paste process. More-recent investigations have shown how recombination triggers DNA synthesis and why the coupling of these two processes is important.
Domain Motions in Bacteriophage T4 Lysozyme: AComparison Between Molecular Dynamics and Crystallographic Data B.L. de Groot, 1S. Hayward, D.M.F vanAalten,2 A.Amadei, and H.J.C. Berendsen * 1Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, the University of Groningen, Groningen, The Netherlands Biol 3400 Tortora et al - Chap 13 2 4. Enveloped viruses - some viruses are further surrounded by a membrane envelope o membrane (phospholipids, proteins and carbohydrates) layer surrounding the capsid o membrane does not function like the membrane of a living organism o proteins are typically virus-specific and coded by the virus genome. May be important in attachment of the virion or release ... Origin. One type of mutation in the T4 bacteriophage identified by researchers in phage genetics by the 1950s was known as r (for rapid), which caused the phage to destroy bacteria more quickly than normal.These could be spotted easily because they would produce larger plaques rather than the smaller plaques characteristic of the wild type virus. Through genetic mapping, the researchers had ... Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period. This is in contrast to the time course of recombination observed in wild-type, lysis-inhibited, or lysis-defective (gene t defective ... T4 bacteriophage (phage)-infected cells show a marked increase in latent-period length, called lysis inhibition, upon adsorption of additional T4 phages (secondary adsorption). Lysis inhibition is a complex phenotyperequiring the activity ofat least six T4genes. Twobasic mysteries surroundourunderstandingof t4 bacteriophage pdf Its easy to maintain and breed T4 bacteriopage in a laboratory.Bacteriopage grows in the presence of living susceptible bacteria. In many but not all cases the growth of page leads finally to a lysis of the bacterial ceils, a. The Evergreen State College, Olympia, Washington 98505. bacteriophage typing pdf PDF; OCR (Text) Title: Mutagenesis by Acridines in Bacteriophage T4 Description: In this lecture Crick provided an account of genetic experiments conducted with Leslie Barnett, Sydney Brenner, and Richard Watts-Tobin in 1961. In these experiments they used acridine mutants of viral DNA (mutants produced by chemicals of the acridine type, such ... Bacteriophage, any of a group of viruses that infect bacteria. Bacteriophages were discovered independently by Frederick W. Twort in Great Britain (1915) and Felix d’Herelle in France (1917). Thousands of varieties of phages exist. Certain types serve key roles in laboratory research.
Enterobakterijski fag T4 je bakteriofag koji može da inficira bakteriju E. coli. Njegov dvolančani DNK genom je oko 169 kbp dugačak i sadržan je u ikosaedarskoj glavi, poznatoj kao kapsid.T4 je relativno veliki fag, aproksimativno 90 nm u širok i 200 nm dugačak (većina fagova ima dužinu u opsegu od 25 do 200 nm). Vlakna njegovog repa omogućavaju vezivanje za ćeliju domaćina. The bacteriophage T4 rnh gene encodes T4 RNase H, a relative of a family of flap endonucleases. T4 rnh null mutations reduce burst sizes, increase sensitivity to DNA damage, and increase the frequency of acriflavin resistance (Acr) mutations. Because mutations in the related Saccharomyces cerevisiae RAD27 gene display a remarkable duplication mutator phenotype, we further explored the impact ... The adsorption specificity of bacteriophage T4 is determined by genes 12 and 37, encoding the short tail-fibers (STF) and the distal part of the long tail-fibers (LTF), respectively. Both are trimeric proteins with rod domains made up of similar tandem quasi-repeats, approximately 40 amino acids long. Molecular Biology of Bacteriophage T4 highlights the value of this biological system as a research and teaching tool. The book is a sequel to the 1983 edition and is organized into six major sections: DNA metabolism, regulation of gene expression, phage morphogenesis, structure and function of selected proteins, host-phage interactions, and experiments in T4 molecular genetics. T4 (T4r+) E. coliB E. coliC Wild type; rapid lysis. Less sensitive to UV radiation than T2. T4r E. coliB Rapid lysis. Mutant of T4r+. Non-reverting plaque morphology. ... When finished with your bacteriophage, please dispose of it in one of the following ways: • Use a 20% bleach solution for 10 minutes (ensure the culture does not open until ... Bacteriophage T4 Gene Expression EVIDENCE FOR TWO CLASSES OF PREREPLICATIVE CISTRONS* (Received for publication, December 22, 1972) PATRICIA 2. O’FARRELL AND LAWRENCE M. GOLD From the Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80302 SUMMARY The antibiotic rifampicin has been used in ... To reveal the structure of penultimate DNA intermediates in T4 bacteriophage recombination, resolution of which produces free recombinant molecules, a single-burst analysis of the recombinant progeny was made in multifactor crosses, enabling one to determine quantitatively the different recombinants generated by one or two exchanges within the same chromosome segment. Phage T4 has many optional homing endonuclease genes similar to segF, whereas similar endonuclease genes are relatively rare in other members of the T-even family of bacteriophages. We propose that the general advantage enjoyed by T4 phage, over almost all of its relatives, is a cumulative effect of many of these localized events.